Genetic basis of Dermatomyositis - risk can be determined through haplotype testing, focusing on three loci: PAN2, MAP3K7CL, and DLA-DRB1 (the latter is the “DRB1 risk allele”, often DLA-DRB1*002:01). Dogs are genotyped for haplotypes (combinations of risk and non-risk alleles) at these three loci. Genotypes with two copies of the DLA-DRB1*002:01 haplotype (“CC”), especially in combination with risk alleles from PAN2 and MAP3K7CL (“AABBCC”), markedly increase susceptibility to DMS. Risk levels are classified as low, moderate, or high based on the genotype combination across all three loci, not just DRB1 alone.

Pathophysiology - is a complex autoimmune disease caused by interactions among genetic risk haplotypes and environmental triggers (infection, vaccination, stress).Dogs with high-risk haplotypes develop skin lesions (hair loss, crusts, ulcers) over pressure points and facial muscles, progressing to muscle inflammation and weakness—especially in Collies, where swallowing and gait can be affected.

Complications - High-risk dogs are prone to recurrent or progressive skin ulcers, muscle wasting, and long-term mobility or swallowing difficulties. If not managed, complications may include secondary infections, chronic pain, and severe quality-of-life impairment or death

Why This Matters to Breeders and Vets - Breeders armed with haplotype results can plan crosses that minimize the likelihood of producing affected puppies, encouraging genetic health and breed sustainability. Veterinarians can provide owners with individualized risk assessments and disease surveillance, improving early detection and intervention in at-risk dogs. Haplotype testing enables more precise risk stratification than testing for a single allele alone

Our single test processing service provides comprehensive and reliable testing for a variety of sample types, including cheek swab, blood or tissue samples. Each sample undergoes a meticulous testing process to deliver accurate results. These tests are excluded from the Turnaround Time Guarantee.

• Blood Samples: Collected in EDTA tubes by a licensed veterinarian to ensure high-quality DNA extraction and optimal test accuracy.
• Tissue Samples or Biopsies: Collected by a licensed veterinarian and stored in ethanol to preserve the sample for advanced testing purposes, ensuring flexibility and reliability for specialised testing needs.

These tests and sample types are specifically required as they are conducted as single stand-alone tests. Due to their scientific complexity and the need for precise methodologies, they are processed independently to ensure the highest accuracy and reliability. This service is ideal for veterinarians, breeders, and pet owners seeking insights into a specific genetic condition or trait, while meeting the stringent requirements for these advanced, complex tests.